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Endocrinology, Vol 111, 1743-1745, Copyright © 1982 by Endocrine Society

ARTICLES

Relaxin alters rat uterine myosin light chain phosphorylation and related enzymatic activities

K Nishikori, NW Weisbrodt, OD Sherwood and BM Sanborn

Uteri from estrogen-primed rats were suspended isometrically, contracted by 0.28 microM prostaglandin F2 alpha, and exposed to 1 microgram/ml purified porcine relaxin for 1 and 10 min. Relaxin treatment for 10 min, but not for 1 min, resulted in a significant decrease in the activity of myosin light chain kinase (MLCKase). Calcium-activated ATPase activity of a crude actomyosin fraction was also decreased by treatment with relaxin for 10 min. Relaxin treatment (10 min) also decreased the relative amount of phosphorylated myosin 20,000 dalton light chains. These effects were consistent with the degree of inhibition of uterine contractions by relaxin. The data suggest that relaxin may inhibit uterine contractile activity by decreasing MLCKase activity and, in turn, myosin phosphorylation and ATPase activity.




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Copyright © 1982 by The Endocrine Society