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Endocrinology, Vol 110, 1459-1461, Copyright © 1982 by Endocrine Society

ARTICLES

TSH stimulates 32P-labeling of thyroid nuclear HMG 14, a protein associated with actively transcribed chromatin

E Cooper, RJ Palmer and SW Spaulding

Thyroid slices were incubated with 32Pi with or without TSH. 32P- labeling of acid-soluble nuclear proteins was then examined by two- dimensional polyacrylamide gel electrophoresis and autoradiography. We found that TSH enhanced the labeling of the high mobility group protein HMG 14, a protein that is preferentially associated with actively transcribed chromatin. This observation suggests that changes in HMG 14 phosphorylation may be involved in mediating TSH-induced effects on the structure and function of active chromatin.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1982 by The Endocrine Society