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Endocrinology, Vol 110, 674-676, Copyright © 1982 by Endocrine Society

ARTICLES

Preparation of a biologically active rat prolactin devoid of antidiuretic activity

RA Adler, M Marceau, DW Borst, HW Sokol and WJ Culp

Rat prolactin (NIH rPRL-B2) was purified using Sephadex chromatography and isoelectric focusing. SDS-gel electrophoresis of the original material showed a major band of 23K daltons, as well as several minor bands; the purified prolactin had a single 23K band. The original material contained 33 pg of immunoreactive vasopressin per 0.1 micrograms of material; vasopressin was not detectable in the purified material by either RIA or bioassay. The purified preparation had complete biological activity in a mammary gland bioassay and a receptor binding assay.


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R. Harlan, B. Shivers, and D. Pfaff
Midbrain microinfusions of prolactin increase the estrogen-dependent behavior, lordosis
Science, March 25, 1983; 219(4591): 1451 - 1453.
[Abstract] [PDF]


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Copyright © 1982 by The Endocrine Society