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Endocrinology, Vol 102, 1262-1268, Copyright © 1978 by Endocrine Society

ARTICLES

Vitamin D and cartilage. I. In vitro metabolism of 25- hydroxycholecalciferol by cartilage

M Garabedian, MB Du Bois, MT Corvol, E Pezant and S Balsan

In the present work, the capacity of cartilage to metabolize 25- hydroxycholecalciferol was investigated. Cartilage preparations from growth plate, articular surface, rib, scapula, and ear were isolated from 3-week-old normal rabbits and chickens. Each tissue was separately incubated with tritiated 25-hydroxycholecalciferol (, x 10(-9) M) for 1- 24 h. Incubations of kidney and muscle were performed simultaneously for comparison. Similarly, cultured chondrocytes isolated from rabbit growth plate and articular cartilage were incubated for 1 or 20 h in medium free of fetal calf serum. After methanol-chloroform extraction of tissues, cells, and their respective media, chloroform phases were chromatographed on Sephadex LH-20 columns. The results show that kidney and cartilage are able to convert 25-hydroxycholecalciferol into a derivative which migrates in the 24,25-dihydroxycholecalciferol region. Cartilage tissue previously boiled is unable to metabolize 25- hydroxycholecalciferol. The conversion of 25-hydroxycholecalciferol occurs with all types of cartilage and is also observed in incubations of cultured chondrocytes. In the latter, the polar 25- hydroxycholecalciferol derivative is detected as early as 1 h after addition of 25-hydroxycholecalciferol. Two findings suggest that the polar derivative of 25-hydroxycholecalciferol produced by cartilage is 24,25-dihydroxycholecalciferol: 1) the cartilage derivative and 24,25- dihydroxycholecalciferol (synthetic and biosynthetic) comigrate during Sephadex LH-20 and high liquid pressure chromatography; and 2) both the cartilage derivative and 24,25-dihydroxycholecalciferol are sensitive to periodate treatment.


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B.D. Boyan, V.L. Sylvia, D.D. Dean, F. Del Toro, and Z. Schwartz
DIFFERENTIAL REGULATION OF GROWTH PLATE CHONDROCYTES BY 1{alpha},25-(OH)2D3AND 24R,25-(OH)2D3 INVOLVES CELL-MATURATION-SPECIFIC MEMBRANE-RECEPTOR-ACTIVATED PHOSPHOLIPID METABOLISM
Crit. Rev. Oral. Biol. Med., March 1, 2002; 13(2): 143 - 154.
[Abstract] [Full Text]


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