help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dannies, P. S.
Right arrow Articles by Tashijian, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dannies, P. S.
Right arrow Articles by Tashijian, A. H., Jr

Endocrinology, Vol 101, 1151-1156, Copyright © 1977 by Endocrine Society

ARTICLES

Anti-estrogenic compounds increase prolactin and growth hormone synthesis in clonal strains of rat pituitary cells

PS Dannies, PM Yen and AH Tashijian Jr

Established clonal strains of rat pituitary cells, GH-cells, responded prior to 1974 to 10(-11) to 10(-8)M 17beta-estradiol by increasing prolactin synthesis 2-fold and decreasing the production of growth hormone to between 20 and 70% of control values. In experiments in 1975- 76, these effects of estradiol were no longer statistically significant. Unexpectedly, the estrogen antagonists enclomiphene, CI- 628, tamoxifen, and Lilly 88751 caused increases in both prolactin and growth hormone synthesis at concentrations of about 10(-7) to 10(-6)M after one week of treatment. These increases were prevented by 10(-8)M 17beta-estradiol. Prolactin synthesis remained elevated for at least 11 days after removal of enclomiphene from the culture medium but, in the presence of estradiol, synthesis approached control levels by 11 days. In GH-cells, compounds which are estrogen antagonists in other systems mimic the previously observed effect of estradiol on prolactin synthesis, but have an effect opposite to that of estradiol on growth hormone, namely stimulation of its synthesis.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
T. Miyazaki, Y. Kanou, Y. Murata, S. Ohmori, T. Niwa, K. Maeda, H. Yamamura, and H. Seo
Molecular Cloning of a Novel Thyroid Hormone-responsive Gene, ZAKI-4, in Human Skin Fibroblasts
J. Biol. Chem., June 14, 1996; 271(24): 14567 - 14571.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1977 by The Endocrine Society